Ancillary Reagents

Description

Histo-Plus PRO is positively charged slides which are colored-frosted on one end. Histo-Plus PRO- Positive charged slides provide an ideal solution for the adhesion of tissue sections during the staining process. These slides are specially treated to possess a positive charge, which facilitates the binding of negatively charged tissue components. Histo-Plus PRO - Positive charged slides enhance the efficiency and reliability of IHC experiments by ensuring optimal specimen retention and minimizing background staining. With their superior tissue adhesion properties, these slides contribute to improved slide quality, sharper staining results, and increased reproducibility.

Description

The PAP pen is an essential tool in immunohistochemistry (IHC) procedures used to create hydrophobic barriers on glass slides. It helps prevent the mixing or spreading of reagents between adjacent tissue sections, ensuring accurate and reliable results. The PAP pen is typically a water-resistant marker pen containing a hydrophobic compound. When applied to a glass slide, it forms a repellent coating that acts as a barrier. This coating allows for precise demarcation around tissue sections using lines or circles, confining the liquid reagents within the designated areas. It involves drawing lines or circles directly onto the glass slide around the tissue sections. This process ensures that the liquid reagents stay localized and do not contaminate neighbouring sections. By creating distinct boundaries, the PAP pen improves the efficiency and accuracy of the IHC process, allowing for reliable and reproducible staining results. PAP pen is a crucial tool in IHC, providing a practical and effective means of creating hydrophobic barriers on glass slides. By confining reagents to specific areas, it ensures accurate staining and helps maintain the integrity of tissue sections throughout the IHC process. Other than IHC, it is also used in Immunocytochemistry, ISH & FISH applications.