Immunohistochemistry (IHC)

Description

Poly-HRP Detection Kit (Two-Step) is a versatile and efficient immunohistochemistry (IHC) detection system used for the visualization of target antigens in tissue samples. This kit employs a two-step approach, utilizing a polymer-conjugated horseradish peroxidase (Poly-HRP) to amplify the signal generated by the primary antibody. Poly-HRP system provides excellent sensitivity, allowing for the detection of low-abundance antigens and improving the overall signal-to-noise ratio. It is compatible with various sample types and antibody species, making it suitable for diverse research and diagnostic applications. Poly-HRP Detection Kit (Two-Step) offers a reliable and convenient solution for IHC staining, facilitating accurate and detailed analysis of tissue markers. This kit streamlines the detection process, saving time and effort while delivering consistent and reproducible results.

Description

Wash Buffer for IHC (Immunohistochemistry) is an essential component of the staining process in histology laboratories. This specially formulated buffer, composed of phosphate-buffered saline, ensures effective and efficient washing of tissue sections or cells during the IHC procedure.

PBS Wash Buffer aids in the removal of unbound antibodies, reagents, and other unwanted substances, while preserving the integrity of the specimen. Its optimized pH and salt concentration help maintain optimal conditions for antibody-antigen interactions, leading to accurate and reliable staining results.

PBS Wash Buffer for IHC is a trusted choice for researchers and pathologists seeking high-quality immunohistochemical analysis.

Description

AR6 - antigen retrieval solution (Low pH) is a citra based buffer, widely used in research and diagnostic laboratories to maximize the sensitivity and accuracy of immunohistochemical analysis, enabling improved visualization and interpretation of tissue markers. Antigen retrieval is a crucial step in immunohistochemistry (IHC) that enhances the detection of specific antigens in tissue samples. This technique involves subjecting the tissue sections to heat or enzymatic treatment to unmask and recover antigens that may be masked or damaged during fixation. By employing antigen retrieval, the accessibility of antigens to antibodies is improved, resulting in enhanced staining intensity and sensitivity in IHC. The process involves carefully optimizing the retrieval method, including choice of retrieval solution, temperature, and duration, to ensure optimal antigen recovery while preserving tissue morphology. AR6 - antigen retrieval solution plays a vital role in maximizing the accuracy and reliability of IHC staining, enabling researchers and pathologists to obtain more accurate and meaningful results. PBS Wash Buffer for IHC is a trusted choice for researchers and pathologists seeking high-quality immunohistochemical analysis.

Description

AR9 - antigen retrieval solution (High pH) is a Tris based buffer, widely used in research and diagnostic laboratories to maximize the sensitivity and accuracy of immunohistochemical analysis, enabling better visualization and interpretation of tissue markers. Antigen retrieval is a crucial step in immunohistochemistry (IHC) that enhances the detection of specific antigens in tissue samples. This technique involves subjecting the tissue sections to heat or enzymatic treatment to unmask and recover antigens that may be masked or damaged during fixation. By employing antigen retrieval, the accessibility of antigens to antibodies is improved, resulting in enhanced staining intensity and sensitivity in IHC. The process involves carefully optimizing the retrieval method, including choice of retrieval solution, temperature, and duration, to ensure optimal antigen recovery while preserving tissue morphology. AR9 - antigen retrieval solution plays a vital role in maximizing the accuracy and reliability of IHC staining, enabling researchers and pathologists to obtain more accurate and meaningful results.

Description

Histo-Plus PRO is positively charged slides which are colored-frosted on one end. Histo-Plus PRO- Positive charged slides provide an ideal solution for the adhesion of tissue sections during the staining process. These slides are specially treated to possess a positive charge, which facilitates the binding of negatively charged tissue components. Histo-Plus PRO - Positive charged slides enhance the efficiency and reliability of IHC experiments by ensuring optimal specimen retention and minimizing background staining. With their superior tissue adhesion properties, these slides contribute to improved slide quality, sharper staining results, and increased reproducibility.

Description

The PAP pen is an essential tool in immunohistochemistry (IHC) procedures used to create hydrophobic barriers on glass slides. It helps prevent the mixing or spreading of reagents between adjacent tissue sections, ensuring accurate and reliable results. The PAP pen is typically a water-resistant marker pen containing a hydrophobic compound. When applied to a glass slide, it forms a repellent coating that acts as a barrier. This coating allows for precise demarcation around tissue sections using lines or circles, confining the liquid reagents within the designated areas. It involves drawing lines or circles directly onto the glass slide around the tissue sections. This process ensures that the liquid reagents stay localized and do not contaminate neighbouring sections. By creating distinct boundaries, the PAP pen improves the efficiency and accuracy of the IHC process, allowing for reliable and reproducible staining results. PAP pen is a crucial tool in IHC, providing a practical and effective means of creating hydrophobic barriers on glass slides. By confining reagents to specific areas, it ensures accurate staining and helps maintain the integrity of tissue sections throughout the IHC process. Other than IHC, it is also used in Immunocytochemistry, ISH & FISH applications.